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Laboratory Practicum for Molecular
and Genetic Epidemiology
EPI 223 Spring 2009 (1 unit)
Course Director: Joe Wiemels, PhD
Associate Professor, Department of Epidemiology & Biostatistics
| OBJECTIVES |
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This course introduces practical aspects of the generation
of molecular and genetic data from human clinical specimens, including
from blood, the oral cavity, and pathological specimens. Working in the
Laboratory for Molecular Epidemiology, students will develop familiarity
with procedures to obtain/process clinical specimens, prepare and isolate
DNA and RNA, design and execute PCR assays, generate genotype data, execute
and analyze microarrays, and write a laboratory-based protocol.
By the completion of the course, students will:
1. Demonstrate familiarity with the field and laboratory
procedures for collecting, processing, and storing biological specimens
such as cells, plasma, and saliva, including database tracking and quality
control procedures.
2. Become familiar with genetic databases and online tools that are useful in planning and performing laboratory analyses.
3. Explain procedural methods for setting up and performing PCR reactions,
including the planning (calculations), additions of DNA and reagents,
inclusion of proper controls, and operation of thermocyclers.
4. Demonstrate familiarity with post-PCR analytical techniques for diagnosis
of polymorphisms, including electrophoresis, sequencing, RFLP, and "taqman"
procedures.
5. Develop a genotyping protocol for single nucleotide polymorphism(s)
of interest and write a research protocol based on the analysis of these
polymorphism(s) in their own research field.
6. Become familiar with the types and uses of microarrays and associated analytical procedures.
7. Be able to define a use for protein and antibody-based markers in your research, and the newer analytical methods (luminex, mass-spec).
| PREREQUISITES |
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Designing Clinical
Research (Epi 202) and Molecular
and Genetic Epidemiology I (Epi 217). Exceptions to these prerequisites
may be made with the consent of the Course Director, space permitting.
| FACULTY |
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| ENROLLMENT |
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This course is open to a limited number of individuals outside
of the ATCR and Master's programs. Preference is given to UCSF-affiliated personnel.
We regret that auditing is not permitted. To apply for this course please fill
out and submit the application below. Cost and submission information are in
the application.
Application (Word doc, 20KB)
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